Model development and simulating of a spinning cone evaporator : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology at Institute of Technology and Engineering, Massey University, Palmerston North, New Zealand

The idea of milk pre-concentration at the farm has attracted worldwide interest for many years. A new pilot-scale evaporator (called spinning cone evaporator), which can be operated on the farm and has a compact and efficient design, has been developed at Massey University. However, there is a shortage of knowledge on the design, operation and control of this new evaporator. The main goal of this thesis is to develop a dynamic mathematical model in order to better utilize this evaporator and make further developments. This thesis consists of three parts. Firstly, a first-principles model of a pilot scale spinning cone evaporator is developed using the sub-system modelling techniques of the evaporator from the Laws of Thermodynamics and the general mass and energy balances. The model is dynamic and includes the evaporator, the compressor, the condenser and the product transport sections. The system model describes the dynamic relationships between the input variables (cooling water flowrate, M c , speed of compressor, N comp , feed flowrate, M f , feed temperature, T f and mass composition of feed dry matter, W f ) and the output variables (outlet temperature of cooling water, T co , evaporating temperature, T e , mass composition of product dry matter, w p and product flowrate, M p ), Secondly, the evaporator model was implemented using the software package Matlab along with its dynamic simulation environment Simulink. The differential equations for the evaporator model are embedded in a block diagram representation of the evaporator system. The evaporator Simulink model is divided into three levels, the blocks at the top represent the overall model and global constants used in it. The second level contains the individual sub-systems and the bottom level elements within each sub-system. Results of the model verification are satisfactory. Finally, the model validation is presented for both steady state and dynamic comparisons. The product flowrate (except in the case of feed temperature changes) and evaporation temperature can be predicted at a given time, and the outlet temperature of cooling water and product dry matter composition can also be predicted at a steady state. It can be seen that the results predicted using this spinning cone evaporator model, which accounts for the varying concentrate flowrate and evaporation temperature with time, are in good agreement with experimental data. This model provides a valuable tool to predict performance in a spinning cone evaporator and to modify the design parameters

Oblique impact breakage unification of nonspherical particles using discrete element method

Particle breakage commonly occurs during processing of particulate materials, but a mechanistic model of particle impact breakage is not fully established. This article presents oblique impact breakage characteristics of nonspherical particles using discrete element method (DEM) simulations. Three different particle shapes, i.e. spherical, cuboidal and cylindrical, are investigated. Constituent spheres are agglomerated with bridging bonds to model the breakage characteristics under impact conditions. The effect of agglomerate shapes on the breakage pattern, damage ratio, and fragment size distribution is fully investigated. By using a newly proposed oblique impact model, unified breakage master surfaces are theoretically constructed for all the particle shapes under oblique impact conditions. The developed approach can be applied to modelling particulate processes where nonspherical particles and oblique impact breakage are prevailing.</p

Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR

Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive, specific assay for the detection and quantitation of PCV2, we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility, and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from 0.59% to 1.05% in the same assay and from 1.9% to 4.2% in 10 different assays. The assay did not cross-react with porcine circovirus type 1, porcine reproductive and respiratory, porcine epidemic diarrhea, transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies, respectively. Using the established real-time PCR system, 39 of the 40 samples we tested were detected as positive

Research on video enhancement concentration and intelligent monitoring technology in Substation

With the development of intelligent theory of computer vision and the popularization of surveillance cameras, intelligent video analysis and surveillance technology has been widely used. The traditional video monitoring system will produce a large amount of video information in the application process, which is not conducive to the storage and reference of information, and it can not carry out real-time warning, and has poor robustness to the bad environment. In view of these disadvantages, this paper proposes an improved video enhancement and concentration method, which has a good effect in ensuring the video concentration ratio and the target loss rate, and can improve the shortcomings brought by manual reference. Aiming at the problem of real-time early warning, an intelligent video analysis method is proposed to realize the functions of event alarm and detection analysis. It is of great significance to improve the video monitoring management level of the substation and realize the unattended operation of the substation

A Turn-Off Fluorescent Strategy for Calprotectin Detection Based on the Inhibitory Effect of Calprotectin upon the Activity of Zn(&#8545;)-Dependent DNAzyme

Calprotectin (CP) is an established biomarker that allows the noninvasive evaluation of inflammation levels in the gastrointestinal tract of patients with inflammatory bowel disease and is helpful for the diagnosis and management of the disease. Herein, we demonstrate that CP can effectively suppress the activity of 17E DNAzyme (17E) by chelating Zn(&#8545;), which is the cofactor of 17E. As the inhibition efficiency of CP on the DNAzyme is proportional to the concentration of CP, the detection of CP can be readily achieved by assessing the activity of 17E

A Turn-Off Fluorescent Strategy for Calprotectin Detection Based on the Inhibitory Effect of Calprotectin upon the Activity of Zn(Ⅱ)-Dependent DNAzyme

Calprotectin (CP) is an established biomarker that allows the noninvasive evaluation of inflammation levels in the gastrointestinal tract of patients with inflammatory bowel disease and is helpful for the diagnosis and management of the disease. Herein, we demonstrate that CP can effectively suppress the activity of 17E DNAzyme (17E) by chelating Zn(Ⅱ), which is the cofactor of 17E. As the inhibition efficiency of CP on the DNAzyme is proportional to the concentration of CP, the detection of CP can be readily achieved by assessing the activity of 17E

Characterization of Bonding between Asphalt Concrete Layer under Water and Salt Erosion

The contact state between layers of asphalt pavement not only has a significant effect on the mechanical response of road structure but is also the bottleneck of research on the mechanical behavior of pavement structure at present. In this paper, the effects and laws of different water&ndash;salt entry modes, salt solution concentrations, and temperatures coupling on the contact state between base and surface layers are studied by a 45&deg; inclined shear test. The simulation and verification of each working condition are carried out by ABAQUS (Dassault, Paris, France) the friction coefficient between layers is reversed, and the actual contact state between layers is characterized in order to realize comprehensive evaluation and reasonable expression. The results show that different modes have different effects on contact characteristics. At the same temperature and concentration of the salt solution, bonding of water and salt erosion is the best, followed by direct erosion, with the worst being from bottom to top, and the interlayer bonding condition is weakened with increase in temperature. The relative accuracy of the software simulation and test analysis was as high as 92% and the friction coefficient of the water-free salt erosion test piece was found to be about 0.85 at 25 &deg;C, while after the bottom-up erosion of the 14% salt solution the friction coefficient was found to be about 0.43, which indicates that the corrosion of the water&ndash;salt will have a great effect on the bonding condition between the structural layers of the road

Hepatocellular Carcinoma Cells Induce Regulatory T Cells and Lead to Poor Prognosis via Production of Transforming Growth Factor-β1

Background/Aims: Regulatory T cells (Tregs) are associated with a poor prognosis in hepatocellular carcinoma (HCC). The purpose of the study was to explore the mechanisms of Tregs accumulation in HCC. Methods: We analyzed the frequency of Tregs in HCC by flow cytometry and immunohistochemistry. We also established a transforming growth factor (TGF)-β1-knockdown cell line by lentivirus-mediated RNA interference. Mouse CD4+CD25- T cells were cultured in supernatants from various cell lines. Results: HCC patients had a high frequency of Tregs, and high numbers of Tregs correlated with a poor prognosis. Liver cancer cells induced Treg production by secreting TGF-β1. In vivo experiments indicated that knockdown of TGF-β1 reduced the numbers of Tregs and metastatic nodules in mice. Conclusions: These results indicate that cancer-secreted TGF-β1 may increase Tregs, and TGF-β1 knockdown might impair immunosuppression in the tumor microenvironment by decrease Tregs

Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription

Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively. Herein, we developed a simple, low-background, and label-free method for both T4 PNK activity detection and inhibitor screening by combining a designed ligation-triggered T7 transcriptional amplification system and a crafty light-up malachite green aptamer. Moreover, this method successfully detected PNK activity in the complex biological matrix with satisfactory outcomes, indicating its great potential in clinical practice